High Fidelity (HF) Restriction Enzymes have 100. The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. An E.coli strain that carries the cloned and modified NotI gene from Nocardia otitidis-caviarum (ATCC 14630). NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin. We are excited to announce that all reaction buffers are now BSA-free.
HF enzymes also exhibit dramatically reduced star activity. BstZ17I-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot 10149198. The items we carry in stock are listed in the table below as well as in the NEB UMaine Freezer Program website.To view the entire NEB catalog, visit their website here. High Fidelity (HF) Restriction Enzymes have 100 activity in rCutSmart Buffer single-buffer simplicity means more straightforward and streamlined sample processing. The University of Maine Freezer Program receives a 15 discount on all products available in the New England Biolabs (NEB) catalog. These engineered enzymes have the same specificity as the native enzyme, with the added benefit. The respected leader in the field of restriction enzyme biology, NEB has developed a line of High-Fidelity (HF) Restriction Enzymes. This product is related to the following categories: Restriction Endonucleases: N-O, Time-Saver Qualified Restriction Enzymes Products This product can be used in the following applications: Restriction Enzyme Digestion NotI has a High Fidelity version NotI-HF ® ( NEB R3189 ). For over 35 years, New England Biolabs has been developing innovative solutions for molecular biology applications. coli strain that carries the cloned NotI gene from Nocardia otitidis-caviarum (ATCC 14630). Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.įor details on NEB’s quality controls for restriction endonucleases, visit our Restriction Enzyme Quality page.Īn E. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. HF enzymes also exhibit dramatically reduced star activity. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer single-buffer simplicity means more straightforward and streamlined sample processing. R3138S SalI-HF, 2.000 u 1 t T 52,70 R3140S PstI-HF, 10.000 u 1 t T 57,80 R3142S KpnI-HF, 4.000 u 2 t T 55,25 R3150S PvuI-HF, 500 u 2 t T 62,90 Rack 6 R3156S SacI-HF, 2.000 u 2 t T 53,55 R3182S SphI-HF, 500 u 1 t T 58,65 R3189L NotI-HF, 2.
NotI has a High Fidelity version NotI-HF ® ( NEB #R3189).
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